Establishing Sox10-VENUS transgenic mice utilizing BAC recombination in E. coli for analyses of neural crestopathy
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چکیده
منابع مشابه
Recombineering of BAC DNA for the Generation of Transgenic Mice
The use of BAC plasmids for transgenic gene expression is also gaining popularity over traditional proximal promoter driven transgene expression because the BAC typically contain most, if not all, of the important regulatory elements required to recapitulate endogenous gene expression (Giraldo and Montoliu 2001). Maintaining the coding sequence within its regulatory locus insulates the transgen...
متن کاملExpression of human CAR splicing variants in BAC-transgenic mice.
The nuclear receptor constitutive androstane receptor (CAR) is a key regulator for drug metabolism in liver. Human CAR (hCAR) transcripts are subjected to alternative splicing. Some hCAR splicing variants (SVs) have been shown to encode functional proteins by reporter assays. However, in vivo research on the activity of these hCAR SVs has been impeded by the absence of a valid model. This study...
متن کاملVisualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells
Fibroblast growth factor 5 (Fgf5) has been widely used as a marker for the epiblast in the postimplantation embryo and epiblast stem cells (mEpiSCs) in the mouse, making it valuable for study of differentiation of various tissues and epiblast cells in vivo and in vitro. Here, we report for the first time the generation of Fgf5-P2A-Venus BAC transgenic (Tg) mice and show that the BAC Tg can reca...
متن کاملExpression Analysis of CB2-GFP BAC Transgenic Mice
The endocannabinoid system (ECS) is a retrograde messenger system, consisting of lipid signaling molecules that bind to at least two G-protein-coupled receptors, Cannabinoid receptor 1 and 2 (CB1 and 2). As CB2 is primarily expressed on immune cells such as B cells, T cells, macrophages, dendritic cells, and microglia, it is of great interest how CB2 contributes to immune cell development and f...
متن کاملCorrection: Expression Analysis of CB2-GFP BAC Transgenic Mice
There are multiple errors in S1 Table. Please view the correct S1 Table below. S1 Table. Primer sequences used of the generation of the CB2-GFP BAC and for the amplification the Southern blot probe. Copyright: © 2015 Schmöle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproductio...
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ژورنال
عنوان ژورنال: Juntendo Medical Journal
سال: 2009
ISSN: 0022-6769,2188-2134
DOI: 10.14789/pjmj.55.34